About SNARF 1 pH6.0

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SNARF-1, AM is a cell-permeant pH probe with a pKa of 7.5 after hydrolysis of the acetoxymethyl ester of the probe upon entering live cells. SNARF-1 is a ratiometric indicator sensitive to measuring pH changes between pH 7 and pH 8 and shifts from 580 nm under acidic pH to 640 nm under basic conditions. It also undergoes a shift in its excitation peak between pH6 to pH9 from ~532 nm at pH6 to ~561nm at pH9, but typically this probe is typically used in fluorescent imaging applications with a single excitation peak at 555nm to monitor the dual emission shift.

Dyes similar to SNARF 1 pH6.0 based on emission

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10-Acetyl-37-dihydroxyphenoxazin 5-TAMRA 6-TAMRA alamarBlue Alizarin Red Amplex Red ATTO 550 ATTO 565 ATTO Rho3B Calcein Red-Orange AM CellBrite Steady 550 CellTrace Yellow CF550R CF568 CF568ST CF570 CMA-Chromomycin A3 Cy3.5 CytoTrace Red DsRed2 dsRed DsRed Express dTomato DY-510XL DyLight 549 DyLight 550 eFluor 585NC EMA ExoBrite 560/585 EV Membrane Stain FVS570 GelRed Kaede (red) KikGR1 (red) LipidSpot 488 LIVE 560 Live-or-Dye 568/583 Live-or-Dye NucFix Red MagDot 575 mApple MaxLight 550 MemBrite Fix 568/580 MITO-ID MP (high potential) mScarlet mTangerine MultiDot 575 NeuroVue Red Nile Red NovaFluor Blue 585 NovaFluor Yellow 590 PE PhenoVue 512 Nucleic Acid Stain PhenoVue 577 Lysosomal Stain PhenoVue Cal-590 AM Bright, Calcium Indicator PhenoVue Cal-590 AM, Calcium Indicator PhenoVue Fluor 400LS PhenoVue Fluor 555 Live Cell pHrodo Red PROTEOSTAT-Red Qdot 585 Resazurin Resorufin RFP Rhod-5N (Ca2+ bound) Rhodamine Rhodamine Red-X RealYellow 586 Seta-400-NHS Spark YG 581 Spark YG 593 StarBright Blue 580 StarBright Yellow 575 SureLight RPE TagRFP tdTomato TRITC TSA Vivid 570 VRDye 549 Zombie YG581 Spark PLUS YG581 cFluor YG584 RayBright PE Ethidium Bromide Taxol Janelia Fluor 549 BactoView Dead 570/585 TyraMax 560/580 MultiDot 580 CytoLiner 570/590 ExoBrite 555/575 True EV Membrane Stain NucSpot 568/580 JFX 554, Maleimide iFluor 568